Flow Cytometry_AMNIS ? ImageStreamX Mk II
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AMNIS ? ImageStreamX Mk II Performance characteristics:
2 camera system, delivering images of brightfield, side scatter (SSC) and 9- 10 fluorescent channels.
Laser and bandpass configuration: AMNIS ImageStreamX ???
- ?Objectives: 20x objectives to view larger structures or cells,
- 40x and 60x objectives for the analyses of cells (as small as yeast or bacteria) or for defining intracellular detail of larger cells
- Extended depth of field (EDF) delivering a single in focus image of a structure or cell
- ?High throughput with an imaging rate per magnification as follows:
| ?Magnification | Max acquisition rate |
| 20x | 5 000 cells/sec |
| 40x | 2 000 cells/sec |
| 60x | 1 200 cells/sec |
- ?Automated acquisition from 96 well round bottom plates
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- Manual acquisition from 1.5 ml microcentrifuge tubes or 96 well round bottom plates
- Sample in suspension is required, able to analyse sample volumes between 20-200ul
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- High resolution images and statistics are retrieved in real-time?
- Automated instrument operations which includes start up, calibration and shut down
- Enumeration of events (events/μl), which eliminates the need of using counting beads
Applications
Nuclear Translocation of NFkB, Intracellular molecular trafficking, Immune Synapse, Shape change, Phagocytosis, Autophagy, etc.
?For more information, please contact us?.
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