?Equipment database DNA Sequencer

Covaris S2 Focused ultrasonicator

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Model:                 S2

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Deliver controlled, focused acoustic energy to a range of samples types

Purpose:              DNA and RNA shearing, cell lysis

 

Eppendorf Refrigerated Centrifuge

 
Model:                 5804R

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Refrigerated centrifugation for samples in plate format

Purpose:              Separation of particles from solution according to size, shape, density and viscosity

 

Agilent BioAnalyzer


Model:                 2100

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Chip-based capillary electrophoresis of DNA, RNA and protein

Purpose:              Fragment analysis, quantification, and quality control of DNA, RNA and protein

 

Class II Biological Safety Cabinet

 

Model:                 LabGard ES NU-437S

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Protection against contamination of samples, environment, and personnel

Purpose:              Reduce potential for exposure of product/ personnel to airborne biological agents


 

Tecan liquid handler


Model:                 Freedom evo

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Liquid handling with robotic functionality for general purpose pipetting

Purpose:              Laboratory process automation

 

Qubit 4 Fluorometer


Model:                 4.0

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Detection of fluorescent dyes that bind to specific target molecules

Purpose:              Precision fluorometric quantification of DNA, RNA and protein


 

PerkinElmer LabChip Nucleic Acid Analyzer

 
Model:                 GX Touch 24

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Automated microfluidic capillary electrophoresis

Purpose:              Quantification and sizing of DNA and RNA

 

MiniAmp Thermal cycler


Model:                 MiniAmp

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Programmed temperature regulation in for denaturation and reannealing of DNA

Purpose:              Amplify DNA and RNA


Agilent 2200 TapeStation

 

Model:                 2200

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Automated electrophoresis platform

Purpose:              Sizing, quantification and quality control of RNA and DNA for downstream workflows

 

GeneAmp 9700 PCR system


Model:                 9700

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Programmed temperature regulation in for denaturation and reannealing of DNA

Purpose:              Amplify DNA and RNA


Sage Science Pippen Prep


Model:                 Pippen Prep

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Automated agarose gel electrophoresis

Purpose:              Size selection of nucleic acids for sequencing workflows


NanoDrop spectrophotometer


Model:                 ND-1000

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Spectrophotometric microvolume quantitation of nucleic acids and proteins

Purpose:              Quantify and assess purity of DNA, RNA and proteins


Step One Plus Real-time PCR system

 

Model:                 Step One Plus

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Amplification and fluorometric real-time quantification of double stranded DNA

Purpose:              Closed system genotyping and quantification of amplifying DNA

 

 

Verity Thermal cycler


Model:                 Verity

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Programmed temperature regulation in for denaturation and reannealing of DNA

Purpose:              Amplify DNA and RNA


3730xl DNA Analyser

 

Model:                 3730xl

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             High-throughput capillary electrophoresis with fluorescence detection

Purpose:              Sequencing and fragment analysis including microsatellites, AFLP, SNP analysis

 

3500xl Genetic Analyzer

 

Model:                 3500xl

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Medium-throughput capillary electrophoresis with fluorescence detection

Purpose:              Sequencing and fragment analysis including microsatellites, AFLP, SNP analysis


GeneTitan Multi-Channel instrument


Model:                 GeneTitan

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Microarray processing

Purpose:              Genotyping and expression studies


Ion Personal Genome Machine

 

Model:                 PGM

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Massively parallel DNA fragment sequencing

Purpose:              Targeted DNA and RNA sequencing, copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing


Ion GeneStudio S5 Prime

 

Model:                 Prime

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Massively parallel DNA fragment sequencing

Purpose:              Targeted DNA and RNA sequencing; copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing, exome and transcriptome sequencing

 

Proton Semiconductor sequencer

 

Model:                 Proton

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Massively parallel DNA fragment sequencing

Purpose:              Targeted DNA and RNA sequencing; copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing, exome and transcriptome sequencing

 

Ion S5 Semiconductor Sequencer

 
Model:                 S5

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Massively parallel DNA fragment sequencing

Purpose:              Targeted DNA and RNA sequencing; copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing, exome and transcriptome sequencing


Ion Chef? Instrument

 

Model:                 Chef

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Closed system, robotic liquid handler

Purpose:              Automated library preparation, template preparation, and chip loading for sequencing of ion sphere particles on semiconductor sequencers

AB Library Builder

 

Model:                 Library builder

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Automation of library preparation or DNA extraction protocols

Purpose:              Library preparation for massively parallel sequencing applications or DNA extraction

 

 

Nuaire Glacier -86 Ultra-Low Temperature Freezer


Model:                 Glacier

Location:             Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function:             Ultralow freezer

Purpose:              Preserve samples at ultralow (-80 Degrees) freezing ranges

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